Re: Shark vertebrae

<< Previous Message | Next Message >>
From:Alan Bright <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

I would have thought this would be very simple to section in a capable

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
PE18 6EB

Tel No; 01480 454528
Fax No;01480 456031
Email ;

-----Original Message-----
From: Michelle McCafferty <>
To: <>
Date: Thursday, December 10, 1998 11:00
Subject: Shark vertebrae

>Hi everyone!
> A researcher at the MBL is interested in cutting 100 micron
>sections longitudinally through individual vertebra from the gray shark.
>Being an elasmobranch this will be cartilaginous vertebra.  He has done
>literature searches on the subject and has come up with several options
>for methodology.  The MBL has a vibrating microtome available for him to
>use - which was mentioned in one paper he has - but he is a little at a
>loss as to how his samples should be prepared to cut 100 micron sections.
>This is out of my realm of experience so I am appealing to the Histonet
>gurus for help.  The vertebrae are already fixed and he believes the
>fixative is formalin.  The question - how should the vertebra be prepared
>for cutting on the vibrating microtome?  Should it be embedded, and if so
>in which medium?  Or is it possible to cut the vertebra without embedding
>on this sort of microtome.  I apologize if some of these questions sound
>silly, but like I said I do not have any experience with vibrating
>(other than being told that they are not all Vibratomes!).  Any
>suggestions, recommendations, experiences, etc....will be very much
>appreciated.  I can forward all information to the researcher or have him
>get directly in touch with anyone who would care to discuss specifics more
> As always, many thanks in advance.
> Michelle
>Michelle McCafferty
>Marine Biological Laboratory
>7 MBL Street
>Woods Hole, MA 02543-1015

<< Previous Message | Next Message >>