Re: Rat vertebrate paraffin sections

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From:Karen S Pawlowski <> (by way of histonet)
To:histonet <>
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Hello Clover and everyone,

I don,t know about joints in paraffin, but I always use Harris Hematoxylin
on temporal bones processed in nitrocellulose (Parlodion) according to the
AFIP  manual.

Karen Pawlowski
UT Southwestern Med. Ctr/ UT Dallas
Dallas, TX

On Thu, 10 Dec 1998 wrote:

> Date:  12/10/1998  03:46 pm  (Thursday)
> From:  Clover Daley
> To:  pcu_dom.api:""
> Subject:  Rat vertebrate paraffin sections
> Dear Histonet followers:
> I am working on a protocol for processing zygapophyseal
> joints from L3-S1 of the rat vertebrate. The sections are to
> demonstrate any bridging of articular cartilage in these areas.
> The protocol that I have reviewed is similar to the one in the
> third edition of the AFIP manual, pg. 47-52 for processing
> human temporal bones.
> I would like to process in paraffin after decalcification instead
> of nitrocellulose.
> Questions:
> The procedure requires me to serial section each joint at 50
> microns and stain every tenth one in Ehrlich' Hematoxylin
> with a light green counterstain. Could I substitute Harris
> Hematoxylin?
> Does anyone have information on staining times for sections
> this thick?
> Also, if anyone has done staining to demonstrate cartilage
> bridging different from this, please let me know. I am open for
> any suggestions.
> Thank you,
> Clover Daley
> Palmer Center for Chiropractic Research
> Davenport, Iowa

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