Re:Pregnancy, dioxane, formalin; LN2; RNA protocols
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From: | Louise Burrell <lburrell@pathbox.wustl.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Wed, 9 Dec 1998, Louise Burrell wrote:
> Dear all: Hi! As far as pregnancy and formalin----I would be much moore
> concerned about Xylenes---wear nitrile gloves and have a properly
> fitted (by your health and safety dept) respirator. Back in cave man
> days--the 70's---I grossed in tissues, as well as changed and worked with
> a rotary tissue processor that used DIOXANE!!!!!!! as it's clearing agent.
> I made up formalin from scratch, coverslipped all histology and cytology
> slides, etc. etc. etc--with NO PROTECTION! We did not know all this.
I was
> pregnant-and other than some of the chemical smells nauseating
> me---none of the pathologists seemed worried about me or my baby. My son
> weighed almost ten lbs with apgars of 9 & 9. He just turned 23 yrs.
> old, is married (1 year) and (just found out) his wife is pregnant. The
> really good news is that all those nasty things did not hurt him
> neurologically---John Alexander Flynn is graduating from Baylor LAW school
> on Feb 6, 1999!! So, other than this being an update to my many histology
> friends whom I may not have been in contact with recently--I am
> bragging!!!!.
> Also, in every situation--assume the worst in histology (use Universal
> Precautions) and protect yourselves for your children and grandchildren's
> sake!! Better safe than sorry, always.
>
> LN2 disposal/evaporation/ and decontamiation
> I go to the Neuro ORs on a regular basis to pick up brain
> specimens--tumors in a dewar's with LN2. I have tried to request surgeons
> to place tissue in a 4mil. thick ziploc plastic baggie--then plop into
> LN2----however, they don't want to. It would help in many ways-but,
> instead I clean with 20-50% clorox (sodium hypochlorite) after I have to
> fish out all the tiny particles---let's not forget about JCD--whoops--CJD
> today!!!!!!! A very nasty dementing disease proven to afflict Histotechs
> in the past--victims usually do not survive for more than 2-5 yrs. after
> exposure!! BE CAREFUL!! I usually let LN2 evaporate under the hood
> first. Remember,in order to activate sodium hypochlorite--you need to add
very hot H2O. Then, let dewar's sit in that mixture for awhile and
then rinse well
> with more hot, hot H2O and then turn upside down on a pipette tip boxlid
> to drain well---then replace in my -80* freezer for the next case.
>
> If I may be of any more assistance- please write.
>
> Michelle Down ---I am having trouble faxing to you---It will be
> accomplished as soon as my fax cooperates-
-Thanx and HAPPY HOLIDAYS TO
> YOU ALL!!!
>
> Mary Vaughan-where are you? Miss our chats.
>
> See y'all next year--byeeee
> With warmest wishes and finally off my" soapbox"
>
> Louise "Beezie" Burrell
> Washington University School of Medicine
> Cancer Center Tumor Repository Core Laboratory Facility
> St. Louis, MO. 63110
> (314) 454-7615 phone
>
>
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