Re: FW: bcl-2

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From:Catherine Bennett <> (by way of histonet)
To:histonet <>
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Joyce Weems:

One of our colleagues has been doing BCL-2 IHC successfully for some time now
in rat tissues using the BCL-2 antibody from PharMingen (San Diego, CA).  He
just got the paper published and the methods section is all very clear.
Briefly, the tissues were fixed in Zinc Formalin, decalcified in formic acid,
and stained using somewhat standard IHC with an added antigen retrieval step.

The paper is "Expression of the BCL-2 Protein in Nasal Epithelia of F344/N Rats
during Mucous Cell Metaplasia and Remodeling."  by J. Tesfaigzi, J. Hotchkiss,
J. Harkema (1998) in Am. J. Respir. Cell Mol. Biol., Vol 18, p 794-799.

Good luck!

Weems, Joyce (by way of Histonet) wrote:

> I am resending this from last week because I've not had any response!
> J:>)
> >----------
> >From:  Weems, Joyce
> >Sent:  Friday, November 27, 1998 9:34 AM
> >To:    'Histonet'
> >Subject:       bcl-2
> >
> >Hey you experts!
> >
> >What have you found to be the best method of determining overexpression of
> >bcl-2 and bcl-1? Specimen type, etc. PCR, Immunoperoxidase, etc?
> >
> >Thanks for all your help!
> >Joyce
> >

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
212G Food Safety + Toxicology Center
Michigan State University
East Lansing, MI 48824
ph:  517-432-4940
fx:   517-353-9902

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