Date:  12/10/1998  03:46 pm  (Thursday)
From:  Clover Daley
To:  pcu_dom.api:"histonet@pathology.swmed.edu"
Subject:  Rat vertebrate paraffin sections

Dear Histonet followers:
I am working on a protocol for processing zygapophyseal
joints from L3-S1 of the rat vertebrate. The sections are to
demonstrate any bridging of articular cartilage in these areas.
The protocol that I have reviewed is similar to the one in the
third edition of the AFIP manual, pg. 47-52 for processing
human temporal bones.
I would like to process in paraffin after decalcification instead
of nitrocellulose.
Questions:
The procedure requires me to serial section each joint at 50
microns and stain every tenth one in Ehrlich' Hematoxylin
with a light green counterstain. Could I substitute Harris
Hematoxylin?
Does anyone have information on staining times for sections
this thick?
Also, if anyone has done staining to demonstrate cartilage
bridging different from this, please let me know. I am open for
any suggestions.
Thank you,

Clover Daley
Palmer Center for Chiropractic Research
Davenport, Iowa
daley_c@palmer.edu