Dear Dr. Miyagishima,

You do not perform pretreatment such as antigen retrieval or enzyme
digestion on frozen tissue.  Pretreatment is generally used to unmask tissue
due to the affects of the fixative such as formalin.  Frozen tissue would be
destroyed by heating.

April Esquibel
DAKO Corporation
Technical Service Representative
800-400-3256 ext. 5338
amedako@earthlink.net

-----Original Message-----
From: Masayuki Miyagishima [mailto:mmiyagis+@pitt.edu]
Sent: Thursday, December 10, 1998 5:57 PM
To: 'Histology Network'
Subject: heat antigen retrieval


I was suggested to do HEAT ANTIGEN RETRIEVAL for frozen tissue sample.

Does it work for frozen tissue, too?  Do I have to do some modification
on protocol?  I am planning to cover slides with 10 mM sodium citrate
buffer, pH 6.0 and put into micorwave for 10 min.
--
Masayuki Miyagishima, MD
University of Pittsburgh,
Department of Surgery
412-647-2345, and ask the hospital operator to page 3228, please.