RE: formalin

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From:"Hinton, Sandy" <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

I can address practices performed at Parkland Hospital in Dallas, Texas from
1961 on. We made 10% NBF and we did pH each batch that was made up.
Sandy Hinton

> ----------
> From: 	Ms Louise Taylor[]
> Sent: 	Wednesday, December 09, 1998 4:27 AM
> To:
> Subject: 	formalin
> Hello histonetters,
> I am currently engaged on a seriously retrospective project involving
> performing PCR on  paraffin embedded archival tissues some of which
> are more than 30 years old. The variablity of the DNA viability and
> content is astounding. A factor that I can only ascribe to the
> various fixatives used way back then.
> Could some of the older histotechnologists give me some insight into
> what was common practice in say, the late 50's early 60's?
> (I will understand if some of you just say that you are reporting
> hearsay - after all - we don't all want to reveal our age?)
> When did buffered formalin come into vogue? As a mere whippersnapper
> of some 17 years experience - I can recall the unpleasantness of
> trying to make up buffered formalin in 25l quantities - an innovation
> and a great pain to a raw recruit.!
> I will appreciate any insight into what really happened
> many thanks
> Louise Taylor
> Johannesburg
> South Sfrica

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