RE: To embed or not to embed?
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From: | Cynthia Favara <cfavara@atlas.niaid.nih.gov> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Amy,
I would not recommend this but I can tell you that we have had lots
of embedder problems and one time I had to leave all the cassettes in a
solid block for a week due to vacation and equipment malfunction.
Everything came out fine, can't remember if ther were any immunos.
Cynthia Favara
> ----------
> From: Woodfin, Amy C[SMTP:AWoodfin@peacehealth.org]
> Sent: Monday, December 14, 1998 12:34 PM
> To: 'histonet@pathology.swmed.edu'
> Subject: To embed or not to embed?
>
> Hi folks,
>
> Still on this wonderful RPI...no really it is! The question has come up
> whether it does any harm to the tissue to pull it out of the processor at
> the end of the cycle and let them sit at room temperature for several hours
> until they are embedded. I can't find anything definitive in Carson,
> Sheehan or AFIP. Only references to crystalization of the paraffin. There
> is no mention if reheating the specimens several hours later and THEN
> embedding them would have any ill effect.
>
> I know we've always done it as soon as the processor if finished, but is
> that the only way? Any input?
>
> Thanks!
>
> Amy Woodfin
> St. Joseph Hospital - Pathology
> Bellingham, WA
> (360) 738-6340
>
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