RE: To embed or not to embed?

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From:Cynthia Favara <> (by way of histonet)
To:histonet <>
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	I would not recommend this but I can tell you that we have had lots
of embedder problems and one time I had to  leave all the cassettes in a
solid block for a week due to vacation and equipment malfunction.
Everything came out fine, can't remember if ther were any immunos.
Cynthia Favara

> ----------
> From: 	Woodfin, Amy C[]
> Sent: 	Monday, December 14, 1998 12:34 PM
> To: 	''
> Subject: 	To embed or not to embed?
> Hi folks,
> Still on this wonderful really it is!  The question has come up
> whether it does any harm to the tissue to pull it out of the processor at
> the end of the cycle and let them sit at room temperature for several hours
> until they are embedded.  I can't find anything definitive in Carson,
> Sheehan or AFIP.  Only references to crystalization of the paraffin.  There
> is no mention if reheating the specimens several hours later and THEN
> embedding them would have any ill effect.
> I know we've always done it as soon as the processor if finished, but is
> that the only way?  Any input?
> Thanks!
> Amy Woodfin
> St. Joseph Hospital - Pathology
> Bellingham, WA
> (360) 738-6340

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