Joyce,
I have used the Chemicon antibody on Formalin fixed rat tissue. If you are
interested in the specifics let me know. It did require HIER.
Mary Georger


> ----------
> From: 	Joyce Kotzuk[SMTP:JKotzuk@salud.unm.edu]
> Sent: 	Monday, November 30, 1998 11:50 AM
> To: 	HistoNet@Pathology.swmed.edu
> Subject: 	ChAT and Enkaphalin antibodies
>
> Does anyone know of antibodies for ChAT (or acetylcholine neurons) and
> met-enkaphalin that work well in paraffin embedded human autopsy tissue? I
> have the Chemicon antibodies for these substances, but they only seem to
> work for me using paraformaldehyde fixed, frozen sections. If anyone has
> any suggestions for making these antibodies work in paraffin material,
> that would be very appreciated as well. Thank-you.            Joyce Kotzuk
>                                                                   Univ. of
> New Mexico, Pathology
>
>