RE: ChAT and Enkaphalin antibodies

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From:"Georger, Mary" <> (by way of histonet)
To:histonet <>
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I have used the Chemicon antibody on Formalin fixed rat tissue. If you are
interested in the specifics let me know. It did require HIER.
Mary Georger

> ----------
> From: 	Joyce Kotzuk[]
> Sent: 	Monday, November 30, 1998 11:50 AM
> To:
> Subject: 	ChAT and Enkaphalin antibodies
> Does anyone know of antibodies for ChAT (or acetylcholine neurons) and
> met-enkaphalin that work well in paraffin embedded human autopsy tissue? I
> have the Chemicon antibodies for these substances, but they only seem to
> work for me using paraformaldehyde fixed, frozen sections. If anyone has
> any suggestions for making these antibodies work in paraffin material,
> that would be very appreciated as well. Thank-you.            Joyce Kotzuk
>                                                                   Univ. of
> New Mexico, Pathology

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