PTAH (phosphotungstic acid hematoxylin) -Reply

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From:Susan Meloan <> (by way of histonet)
To:histonet <>
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While we worked on PTAH, we found that by dissolving the hematoxylin, then
ripening with Potassium Permanganate before adding PTA, we could use the
solution within a few hours.  We got better results with Zenker or
Methacarn fixed tissues, but marginal ones with Formalin fixed tissues.
Have not tried any of the newer fixatives such as Zinc or Glyoxal based


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