| From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Since you are fixing with PFA, then doing the frozens, you may want to try overnight staining, rather than 1 hour, and do it at 4C, sometime even room temp works, overnight. Gayle Callis