Michelle's shark bits...
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From: | Francie Gallery <fgallery@neurobio.sunysb.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi, Michelle,
We routinely cut fixed (formalin/gluteraldehyde) rat spinal cord both in
transverse & longitudinal planes on our vibratome; though for our work we
cut 50um sections. Our vibratome is set-up with a cutting-reservoir of 1M
PBS kept chilled to 4C. We use superglue (no lab should be without it!) to
attach the cord in proper orientation to a base-block, then screw-clamp the
base-block in the reservoir...and cut. We remove each section as it comes
off the blade using a paintbrush then store it in a 25-well tissue culture
plate - allowing us to retain serial order as we cut from dorsal to
ventral. You'll probably have to "experiment" with the speed & amplitude
for optimal cartilage -cutting parameters. I hope this helps...if you need
more details, feel free to contact me directly.
~Francie~
** these opinions are my own, etc....please excuse any errors...I'm a
hunt-n-peck typist and not that good a spellar ;) **
Francie Gallery
Histology Department, Suffolk County Medical Examiner's Office,
Hauppauge, NY
Department of Neurobiology & Behavior, SUNY at Stony Brook, Stony
Brook, NY
FGALLERY@neurobio.sunysb.edu
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