Fixation and Staining for Lipid with Sudan Black

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From:"Bourassa, Patricia" <> (by way of histonet)
To:histonet <>
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Hi all!

One of my colleagues wants to stain rabbit aortic tissue for lipid using
Sudan Black.  Has anyone done this?  Would there be any reason why fixation
of the vessel before staining would interfere with the procedure?  I thought
fixation would enhance the staining if anything (preserve morphology!!) but
we wanted to be sure.

Thanks for any info!!

-Patti Bourassa
 Pfizer, Groton

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