Drying slides for ER

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From:Dave Tacha <dtacha@ncal.verio.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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We have been drying slides for ER at 60 degrees versus 37 degrees and
comparing them for ER staining (1D5 clone).  We are observed reduced
staining when drying at 60 degrees.  The longer we dry at 60 degrees the
less staining we observed.  We have concluded that drying overnight 37
degrees is superior to 60 degrees drying for 1-2 hours.

If we have had problems with the tissue staying on the slide, we would
dry slides in a 60 degree oven for 3-4 hours. We observed less staining
intensity at 3-4 hours versus 1 hour.  We had dried some slides
overnight at 60 degrees. 70-80% of the staining was reduced.

Has anyone ever observed this phenomena before?  Let's chat!

David Tacha HTL (ASCP)
Biocare Medical

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