Re: [Histonet] Schiff's reagent

From:Solomon Adebayo



This is a rather late reply to the querry by Andrea.
The shell life and stability of schiff's reagent
depend on the formular and mode of preparation.
we use a modified schiff's reagent with a shell life
of  more than 12 months. It still maintain its potency
and very stable.
Benard Solomon
Pathology Dept.,
Unilorin Teaching Hospital,
Ilorin, Kwara State,
Nigeria
www.histosearch/homepage/benardsolomon
--- Andrea Grantham  wrote:

> I'm using a Schiff's from Newcomer Supply that 
> has a pretty long shelf life. It can be stored at 
> RT and can be used several times.
> 
> We always put the "used" Schiff's into a 
> different bottle as to not contaminate the unused
> solution.
> 
> Andi Grantham
> 
> 
> 
> 
>   At 03:29 PM 12/3/2007, Rene J Buesa wrote:
> >It has to be kept in the refrigerator, and yes, it
> can be used several times.
> >   You can test how is working by adding a few 
> > drops of the Schiff's reagent to NBF, it it 
> > reacts quickly with a magenta color, is working
> OK, if not, discard it.
> >   René J.
> >
> >Taben Hale  wrote:
> >   What is the shelf-life and stability of Schiff's
> reagent? Can it be used
> >multiple times?
> >
> >--
> >Taben M Hale, PhD
> >Postdoctoral Fellow
> >Universite de Montreal
> >Dept Pharmacologie
> >(514)343-6111 ext. 4968
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>
.....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell
> Biology & Anatomy     :
> : Sr. Research Specialist       University of
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