Re: [Histonet] IHC for VIAS

From:"Godfrey Guerzon"



Thanks Patti,

We have tried this method of 30 minutes in a 60degree oven and
overnight at 37-40 degree oven - it gives us better results but still
part of the tissue is falling off.

Thanks.

Godfrey

>>> Patti Loykasek  12/20/2007 5:11 PM >>>
I would recommend some time in a 60-65 degree oven even if you do still
want
to leave them in a 37-45 degree oven overnight.  That's just off the
top of
my head - busy day here.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> We are having problem with tissue falling off the slides when we run
the
> breast panel on the Ventana XT for examination with the VIAS
instrument.
> The PR, ER, Ki67 and Her2 tends to fall off.  We use "plus" slides
and
> place them in 45 degree oven overnight and they still fall of.
> 
> Any suggestions?
> 
> Thanks.
>         
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