Re: [Histonet] CD68

From:"Majid ghoddusi"



While on CD 68 subject, I have been told that this does not work on mouse
tissue. In other words it is not a good IHC marker for mouse macrophages. I
would appreciate advice from people who have successfuly tried this on mouse
tissue.

Thanks,

Majid






*Majid Ghoddusi, DVM, PhD*
*Veterinary Pathologist*
*Comparative Biosciences, Inc.*
*786 Lucerne Drive, *
*Sunnyvale, CA 94085*
*http://www.compbio.com/* 
*Ph: 408-738-9265*
*Fax: 408-738-9278*




On Dec 20, 2007 9:58 AM, Rene J Buesa  wrote:

> IF you have both control and case running simultaneously and there is no
> problem with the instrument (like malfunctioning over one slide and not over
> the other, which is very unlikely) your problem is with your control.
>  I always used tonsils coming from surgey and ususally older than 2 yo
> patients.
>  I used Dako IgMo Ab at 1:1000 with pH6 HIER
>  Try a new control tissue (fresh sections if at all possible).
>  René J.
>
> Cheri Miller  wrote:
>
>
>
>
>
>
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