RE: [Histonet] Re: Lendrum stains

From:"Houston, Ronald"



I fondly remember performing Lendrum's stains in a previous life back
home in Scotland. The color contrasts of many of the techniques remains=0Dunsurpassed as far as tinctorial staining is concerned. 

=0A 

Not so sure why none of the methods really took off in the States;
perhaps you're right, Bob, it may have had to do with the fact that he
got many of the dyes directly from the mills. 

 =0D
Professor Alan C Lendrum was Professor of Pathology at both Glasgow and
Dundee Universities (the latter until 1972 when I believe he retired).

Perhaps John Bancroft, Peter Stoward and/or Richard Horobin would be
able to comment more on Professor Lendrum, if they still subscribe to
Histonet. It is certainly true that he was one of the last
histopathologists who understood the properties of the dyes and the
theory behind the techniques he introduced with his colleagues=2E 

 

His work was true Art in histology; alas a dieing trait (no pun
intended).

 

As far as I am concerned, his MSB (Martius Yellow, Crystal Scarlet,
Soluble Blue) is still the best method for fibrin demonstration.

 

Ronnie

 

 

Ronnie Houston, MS, HT(ASCP)QIHC=0D
Anatomic Pathology Manager

Nationwide Children's Hospital

700 Children's Drive

Columbus, OH 43205
=0D(614) 722 5465

Ronald.Houston@NationwideChildrens.org=0D
Columbus Children's Hospital is now Nationwide Children's Hospital

www.NationwideChildrens.org

 

 
=0A
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Friday, January 04, 2008 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Lendrum stains

 

Sharon E. Willman (where?) asks for information about "Lendrun" and

Fraser stains, she doesn't say for what.

 

A.C. Lendrum, I was told back around 1970, was one of the last

histologists who understood textile dyes. Apparently there were a lot

of small factories making obscure dyes in Scotland back then, and he

knew all of these single malt dye-makers and collected numerous

samples. His eclectic habits make it difficult to identify the dyes he

used, let alone obtain and use them. That and the invention of

immunohistochemistry probably consign most of his work to oblivion.

=0A 

Lendrum's "Obadiah" stain (he was fond of such fanciful names) for

fibrin was in use in a research lab at Cornell Medical Center on east

68th street in New York City when I was there briefly in 1968.

Stainsfile gives the following reference: Lendrum, A. C., et. al.

(1962) "Studies on the character and staining of fibrin." Journal of

Clinical Pathology, v. 15, p=2E 401. [this is a British journal, not the

AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or

polar brilliant red BN.

 

John Kiernan - Dick Dapson - Mike Titford - other geezers on this list

- do you know anything more about A.C. Lendrum? He must have been an

interesting guy!
=0A
 

Bob Richmond

Samurai Pathologist

=0AKnoxville TN

 

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