I used to use a pressure cooker but found it to be very harsh.  I had a lot of trouble getting the sections to stay on the slide.  For the last several years I'v use a $40 Black and Decker rice steamer.  Slower than the pressure cooker but it always works and no problems with the sections.  You might also look into microwave retrieval.  I tried it a few times and it worked fine.

I steam them for 20 minutes with a 20 minute cooling afterward.  That gives me plenty of time to make up my buffer and set up my run.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar@lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee
Fish
Sent: Thursday, January 03, 2008 3:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pressure cooker question


We are searching for a cheap but reliable way to do antigen retrieval.  Is
anyone out there using an electric pressure cooker?  And, are the ordinary
household appliances working for you?
Thanks in advance for your help.
Jo Dee
 

~~Jo Dee Fish~~
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail:   jfish@gladstone.ucsf.edu

Mailing address:  
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

 
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