RE: [Histonet] ED1 staining in rat

From:"Linke_Noelle"



That's a pretty easy one to get working....

ED1 from Serotec (cat# MCA341R), 1:400 dilution, citrate pretreatment (this Ab is pretty strong, may work without it) 1 hour incubation.

Noelle

NoŽlle Linke, MS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich
Sent: Monday, December 17, 2007 10:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ED1 staining in rat

Does anyone have an IHC protocol for ED1 staining in formalin-fixed
paraffin sections of rat tissue (specifically rat brain)? I've noticed
several mouse anti-rat antibodies available, but I'm not really sure
where to start. Any suggestions would be much appreciated.

 

~Michele


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