Good Morning all, I was wondering if anyone had suggestions on an antibody
to detect T cells (only, no B cells) in formalin fixed, paraffin embedded
rat thymus and spleen. I have a Pan T Cell marker that is working well in
the spleen, but so-so in the thymus, so I think that it is only staining the
mature T cells, and not immature cells (which of course, is what we also
need to see). I've been reading up alot on T cells and trying to figure this
out (CD3, CD4, CD8, TCR, lions, tigers and bears - Oh My!), but I fear our
deadline for getting staining done is shorter than the time I have to
educate myself properly!
Any suggestions would be greatly appreciated!
Kristen Broomall, HT (ASCP)
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