[Histonet] RE: ED1 staining in rat

From:"Wynn, Carmen"



Hello Michele,

I have also had good results with the AbD Serotec's mouse anti-rat CD68
(ED1) in rejected grafts of FFPE rat heart and kidneys at 1:100
dilution.  I used Trypsin Grade II (sigma cat#7168) for 30 minutes at
37C.  I didn't have to use any HIER but as Noelle stated before...it is
a pretty hardy antibody.

Carmen Wynn, M.S., Senior Scientist
Astellas Research Institute of America, LLC. (ARIA)
Illinois Science and Technology Park
8045 Lamon Ave
Skokie, IL 60077
Direct: 847-933-7419
Main: 847-933-7400
Fax: 847-933-7401 


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Sent: Tuesday, December 18, 2007 12:18 PM
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Subject: Histonet Digest, Vol 49, Issue 19

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Today's Topics:

   1. Bouin's fixation for mouse nasal passages (Helen E Johnson)
   2. ED1 staining in rat (Michele Wich)
   3. Desmin (Perry, Samuel)
   4. RE: ED1 staining in rat (Linke_Noelle)
   5. AW: [Histonet] Desmin (Gudrun Lang)
   6. Cold Plate (Piche-Grocki, Jessica)
   7. Re: Schiff's reagent (Solomon Adebayo)


----------------------------------------------------------------------

Message: 1
Date: Mon, 17 Dec 2007 13:32:54 -0500
From: Helen E Johnson 
Subject: [Histonet] Bouin's fixation for mouse nasal passages
To: histonet@lists.utsouthwestern.edu
Message-ID:
	

	
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
      How long should mouse nasal passages be fixed in Bouin solution
before cutting in & processing?
                                                           Helen Johnson
(hej01@health.state.ny.us)


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------------------------------

Message: 2
Date: Mon, 17 Dec 2007 12:48:38 -0600
From: "Michele Wich" 
Subject: [Histonet] ED1 staining in rat
To: 
Message-ID:
	
<2264717ADC396742A0FF0AAB674F9A0D486B65@7THWAVE-SERVER.7thwave.local>
Content-Type: text/plain;	charset="us-ascii"

Does anyone have an IHC protocol for ED1 staining in formalin-fixed
paraffin sections of rat tissue (specifically rat brain)? I've noticed
several mouse anti-rat antibodies available, but I'm not really sure
where to start. Any suggestions would be much appreciated.

 

~Michele


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------------------------------

Message: 3
Date: Mon, 17 Dec 2007 13:24:14 -0500
From: "Perry, Samuel" 
Subject: [Histonet] Desmin
To: 
Message-ID:
	
Content-Type: text/plain; charset="us-ascii"

Hi All,

I am trying to perform IHC staining using the Dako envision+ kit on
mouse and
human tissue.  I am looking for an anti-desmin antibody which has been
well
published and characterized in the past.  There seems to be several
rabbit
polyclonal antibodies out there by abcam, anyone have luck with these?
I look
forward to hearing peoples responses.

 

Thanks -Sam Perry

 

Dana Farber Cancer Institute

Research Technician

 


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------------------------------

Message: 4
Date: Mon, 17 Dec 2007 10:58:01 -0800
From: "Linke_Noelle" 
Subject: RE: [Histonet] ED1 staining in rat
To: "Michele Wich" ,
	
Message-ID:
	
<5C3DA4BE34AA0641BAA10A7C1478B60527CFED@IRMAIL132.irvine.allergan.com>
Content-Type: text/plain;	charset="iso-8859-1"

That's a pretty easy one to get working....

ED1 from Serotec (cat# MCA341R), 1:400 dilution, citrate pretreatment
(this Ab is pretty strong, may work without it) 1 hour incubation.

Noelle

Nolle Linke, MS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele
Wich
Sent: Monday, December 17, 2007 10:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ED1 staining in rat

Does anyone have an IHC protocol for ED1 staining in formalin-fixed
paraffin sections of rat tissue (specifically rat brain)? I've noticed
several mouse anti-rat antibodies available, but I'm not really sure
where to start. Any suggestions would be much appreciated.

 

~Michele


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may contain information that is legally privileged, confidential or
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------------------------------

Message: 5
Date: Tue, 18 Dec 2007 08:03:57 +0100
From: "Gudrun Lang" 
Subject: AW: [Histonet] Desmin
To: "'Perry, Samuel'" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <001401c84144$29ea9480$eeeea8c0@dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

We use monoclonal mouse Desmin D33 from Dako, 1:100 on Ventana Benchmark
(pH
8 retrieval; 24 min inkubation).

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Perry,
Samuel
Gesendet: Montag, 17. Dezember 2007 19:24
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Desmin

Hi All,

I am trying to perform IHC staining using the Dako envision+ kit on
mouse
and
human tissue.  I am looking for an anti-desmin antibody which has been
well
published and characterized in the past.  There seems to be several
rabbit
polyclonal antibodies out there by abcam, anyone have luck with these?
I
look
forward to hearing peoples responses.

 

Thanks -Sam Perry

 

Dana Farber Cancer Institute

Research Technician

 


The information transmitted in this electronic communication is intended
only
for the person or entity to whom it is addressed and may contain
confidential
and/or privileged material. Any review, retransmission, dissemination or
other
use of or taking of any action in reliance upon this information by
persons
or
entities other than the intended recipient is prohibited. If you
received
this
information in error, please contact the Compliance HelpLine at
800-856-1983
and
properly dispose of this information.


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------------------------------

Message: 6
Date: Tue, 18 Dec 2007 12:40:41 -0500
From: "Piche-Grocki, Jessica" 
Subject: [Histonet] Cold Plate
To: 
Message-ID:
	
<0A57D6AEAE4CBA4A984D27257160A72D01069D22@win03exchange01.wtbyhosp.org>
	
Content-Type: text/plain;	charset="iso-8859-1"

Hi,
Just wanted to say thanks to everyone who shared information on cold
plate QC. Thanks again,
Jessica

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------------------------------

Message: 7
Date: Tue, 18 Dec 2007 09:57:07 -0800 (PST)
From: Solomon Adebayo 
Subject: Re: [Histonet] Schiff's reagent
To: Andrea Grantham ,
	histonet@lists.utsouthwestern.edu
Message-ID: <450981.38163.qm@web36105.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

This is a rather late reply to the querry by Andrea.
The shell life and stability of schiff's reagent
depend on the formular and mode of preparation.
we use a modified schiff's reagent with a shell life
of  more than 12 months. It still maintain its potency
and very stable.
Benard Solomon
Pathology Dept.,
Unilorin Teaching Hospital,
Ilorin, Kwara State,
Nigeria
www.histosearch/homepage/benardsolomon
--- Andrea Grantham  wrote:

> I'm using a Schiff's from Newcomer Supply that 
> has a pretty long shelf life. It can be stored at 
> RT and can be used several times.
> 
> We always put the "used" Schiff's into a 
> different bottle as to not contaminate the unused
> solution.
> 
> Andi Grantham
> 
> 
> 
> 
>   At 03:29 PM 12/3/2007, Rene J Buesa wrote:
> >It has to be kept in the refrigerator, and yes, it
> can be used several times.
> >   You can test how is working by adding a few 
> > drops of the Schiff's reagent to NBF, it it 
> > reacts quickly with a magenta color, is working
> OK, if not, discard it.
> >   Ren J.
> >
> >Taben Hale  wrote:
> >   What is the shelf-life and stability of Schiff's
> reagent? Can it be used
> >multiple times?
> >
> >--
> >Taben M Hale, PhD
> >Postdoctoral Fellow
> >Universite de Montreal
> >Dept Pharmacologie
> >(514)343-6111 ext. 4968
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >---------------------------------
> >Be a better sports nut! Let your teams follow 
> >you with Yahoo Mobile. Try it now.
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
>
.....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell
> Biology & Anatomy     :
> : Sr. Research Specialist       University of
> Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044     
>                :
> : (voice:  520-626-4415)        Tucson, AZ 
> 85724-5044    USA       :
> : (FAX:  520-626-2097)          (email: 
> algranth@u.arizona.edu)       :
>
:...................................................................:
>           
> http://www.cba.arizona.edu/histology-lab.html
> 
> 
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