I am a reserach technician in the lab.
I use Normal Goat Serum (NGS) for immunofluorescent IHC stainig as a blocking (10%). Recently I got a new batch of NGS and used it. Even my reliable primary antibody was negative on the rat brain tissue. I tested old vs new NGS, and old one worked.
Does this happen a lot?
Could someone know about good NGS supplier for the staining?
Thank you for your help.
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