I also think, that the old staining methods have their right to exist and
are an important part of modern histology.
What I referred to in my former question were techniques like the
demonstration and identification of proteins, aminoacids, enzymes, pigments.
For example, I have just Lillie's book (1953) in front of me. I find
protocols for the demonstration of arginin, tryptophan, urea, keratin,
keratohyalin, plasmalogen. Or in Pearses book (1968) ninhydrin, DNA, RNA,
ascorbic acid and so on. There are special fixations or cryotechniques
recommended. And sometimes a translater would be helpfull. Not only from
English to German, but also from trivialnames of reagenses.
Are these methods still in use anywhere? Or survived only those, that can be
done on FFPE-tissue?
Von: Maxim Peshkov [mailto:Maxim_71@mail.ru]
Gesendet: Samstag, 05. Jänner 2008 20:21
Betreff: Re: [Histonet] histochemistry an extinct art?
I also work in clinical histology lab. We do H&E
and SS (up to 20 methods). Art of histochemistry
will be always. Today it is for cost
considerations vs IHC. When modern science moves
upward, routine practice still uses many old
methods with big success. And for comparison
purposes any still use old classical methods.
IHC and HC not will never disturbes to each other
as airplanes, autos and pedestrians and will
helps each other.
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