I had a problem with burnt tissue when I demo'ed a microwave tissue
processor a couple of years ago. I cut the slides and used a HIER method
just like on immunos. Some of the tissue stained better and some diagnosis's
were able to be made, but some were totally lost.
Hope this helps
----- Original Message -----
From: "curt tague"
Sent: Monday, December 10, 2007 9:59 AM
Subject: [Histonet] tissue burnt/dried on processor
> to summarize, we had some of the reagents errantly changed on the tissue
> processor. some of the 100% alc. seem to have been changed with 95% which
> we thing resulted in water in the tissue through the xylene and wax.
> however, the tissue was really dry and brittle.... i would really love to
> resolve this problem rather than tell the clients that their tissue is
> destroyed beyond repair. i have a picture i can forward to you if anyone
> would like to take a look. basically, the epi, on skins specifically, is
> staining really light. almost like we didn't leave it in there long
> enough? pathologists were not happy to say teh least.
> please help, let me know if i can forward the pic.
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