Re: [Histonet] section on overfixed paraffin tissue

From:Geoff McAuliffe



Formalin works slowly so "overfixation" is probably not the cause of the 
problem. Sounds like poor infiltration with wax to me. This could be 
secondary to poor dehydration and/or clearing.

Geoff

FU,DONGTAO wrote:
> Hi, all
>
>  One of our histotechnician met a big problem on sectioning overfixed 
> human and mouse pancreas. The tissues were overfixed in 4%NBF for 
> several weeks before our PI gave to us for paraffin embedding and 
> section. The histotechnician could not get intact pieces of the 
> tissues because the sections started to break when they attach to the 
> water(42-45C) in the waterbath. Does anyone have any experiences on 
> sectioning overfixed tissues? Any suggestions?
>
>  Thank you,
>
> Ann Dongtao Fu MD, Ph.D
> Lab Manager
> Molecular Pathology Core
> Dept. of Pathology
> Lab phone: 352-273-7752
> Lab FAX: 352-273-7755
> Lab address: D11-50
> PO Box: 100275
> 1600 SW Archer Road
> University of Flodrida
> Gainesville, FL 32610
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



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