Re: [Histonet] buffered verses unbuffered EDTA for decalcification for tunel

From:Rene J Buesa

EDTA in itself is a buffered solution once you equilibrate its pH during preparation.
  René J.

Liz Chlipala  wrote:
  I need some help in determining if I need to use buffered or unbuffered
EDTA to decal samples for tunel. I have searched the histonet and there
are some protocols for buffered EDTA, but I have also seen protocols
that the EDTA is unbuffered. Any help is appreciated.

Thanks in advance


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540 

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