Re: [Histonet] Mast cell identification

From:"Jan Shivers"

Dako's anti-Mast Cell Tryptase works great.  I use an HIER retrieval buffer 
that incorporates EDTA for 20 minutes (one of Dako's preparation).

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Leader
University of Minnesota
Veterinary Daignostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108

----- Original Message ----- 
From: "Cindy Robinson" 
To: "histonet" 
Sent: Thursday, December 13, 2007 10:07 AM
Subject: [Histonet] Mast cell identification

Does anyone have experience using DAKO antibody-Mast Cell Tryptase?  We have 
been using giemsa stains but my pathologist has asked me to look for 
something more definitive to identify and stain mast cells.  If anyone could 
offer advice I would appreciate it.


Cindi Robinson, HT (ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes, SD  57049
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