Cheryl,
What helped me previously is using a confocal flourescent scope and I'm assuming at LSU there are many. Looking through a "thick section" with traditional flourescence, you will always be burdened with increased background since you are imaging through the entire section. With confocal, all that total background becomes irrelavent since , depending on the power of your confocal, you can "optically" section through the thick section, a few micron optical slices at a time. And 3D image the results to boot by stacking them depending on the software you have.
Raymond Koelling
PhenPath Labs
Seattle, WA
-------------- Original message --------------
From: Rene J Buesa
> No matter what you do, with thick sections you will always get background noise.
> Try to use thinner sections.
> Ren・J.
>
> Cheryl Crowder wrote:
> Hello - I have a researcher who has done IHC on her tissues with good
> success. Now she is trying to do fluorescent antibodies on thick sections
> to be able to do 3D imaging. I have no experience with this technique. Her
> problem, tremendous background staining. Can anyone give us any tips for
> reducing this staining? Thanking you in advance,
> Cheryl
> Cheryl Crowder, BA, HTL(ASCP)
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