Re: [Histonet] Immunofluorescence

From:Rene J Buesa

No matter what you do, with thick sections you will always get background noise. Try to use thinner sections.
  René J.

Cheryl Crowder  wrote:
  Hello - I have a researcher who has done IHC on her tissues with good 
success. Now she is trying to do fluorescent antibodies on thick sections 
to be able to do 3D imaging. I have no experience with this technique. Her 
problem, tremendous background staining. Can anyone give us any tips for 
reducing this staining? Thanking you in advance,
Cheryl Crowder, BA, HTL(ASCP)

Chief Technologist

Anatomic Pathology

Department of Pathobiological Sciences

School of Veterinary Medicine

Louisiana State University

Skip Bertman Drive

Baton Rouge, LA 70803


FAX: 225-578-9720
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