We have gotten freezing artifact when the isopentane is not cold enough. We charge the isopentane with some dry ice and freezing artifact disappears.  We also wash solution cat# 0103, Zeus Scientific and not PBS.

Jeannette Mitchell

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of eileen dusek
Sent: Tuesday, December 04, 2007 11:04 AM
To: histonet
Subject: [Histonet] fresh kidney bx

Hi Everyone,
  I have been in Histology for a looong time and have met my match. I recently have new duties involving kidney bxs. We receive a formalin, EM, and Michels (Zuess) fixed specimens.
  The Formalin and EM bx are not an issue, the Michels is kicking by butt!
  I transfer the specimen from Michels to PBS, to rinse the fixative, for about 2-3 mins of agitation. After the PBS the specimen goes into the first OCT. This is swirled for another 2-3mins. I repeat this process again.
  My problem is I get specimens that have too much freezing artifact. The structures are "blown up" and not compact.
  I appreciate any suggestions to help the problem         Thanks

  Eileen Dusek


---------------------------------
Looking for last minute shopping deals?  Find them fast with Yahoo! Search.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet