RE: [Histonet] Variation in staining

From:"Kemlo Rogerson"

In the last two days we have noticed a problem with our sections having
areas that do not take up the Hematoxylin. This seems to be geographic
pattern that varies at different levels.  For example one level on the
same slide may show this effect, while the next level will be fine.  The
Eosin is not effected.  We have had no changes in our processing.

Odd problem. I do remember having similar problems of patchy staining
when using a rather 'weak' batch of haematoxylin (I grew my own); the
non patchy nature of the eosin suggests the usual culprit of poor
dewaxing of the slides is not responsible. Have you changed the batch of
Haematoxylin recently? Is it past its sell by date? Try a new batch or
another make.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown 

This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation

Histonet mailing list

<< Previous Message | Next Message >>