RE: [Histonet] Re: Amyloid Control

From:"Hofecker, Jennifer L"

The NSH Quality Control committee does maintain a control bank.  In this
particular case, I actually extracted the original post from Ms. Cornett
and forwarded it to our "librarian".  We operate by members donating to
the bank when they have excess control tissue.  Likewise, if you need a
control block, you can either ask the NSH office, or ask me, or post on
histonet which I try to monitor for requests. There is a request form on
the NSH webpage located on the QC committee page.   We are here to help,
if we can, when you need control blocks. Our librarian is amazing and
responds promptly. 
If anyone has any extra control tissue to donate, please let us know. 
Have a great week.

Jennifer L. Hofecker, HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology 
Nashville, TN
ph. (615)343-0083
fax. (615)343-7089
NSH Quality Control Committee Chair

-----Original Message-----
[] On Behalf Of Bernice
Sent: Wednesday, December 05, 2007 10:29 AM
To: 'Robert Richmond';
Subject: RE: [Histonet] Re: Amyloid Control

When I was in Denver I was told that NSH has a control bank. Did anyone
try them? 

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611

-----Original Message-----
[] On Behalf Of Robert
Sent: Tuesday, December 04, 2007 6:02 PM
Subject: [Histonet] Re: Amyloid Control

Lorraine Cornett in Kingsport TN (right up the road from Knoxville)
asked for and got (yay Histonet) an amyloid control.

She noted that paraffin sections do not last more than a couple of
weeks, and need to be cut fairly fresh.

Everybody has trouble getting amyloid controls - a famous academic
surgical pathologist who first made his reputation with amyloid in
George Glenner's laboratory, told me he didn't have one.

What I don't understand is - amyloidosis is rather easily produced in
some experimental animals by repeated injection of proteins such as
casein. Why isn't experimental animal material offered commercially as
an amyloid control?

Medullary carcinomas of the thyroid usually contain some amyloid, and
I've seen them successfully used as amyloid controls.

There are many other problems with amyloid staining. Congo red staining
requires a polarizing microscope, and many pathologists in small
pathology services aren't allowed to have one of these.

Does anybody have any experience with Anatech's Amyloid Red?

Bob Richmond
Samurai Pathologist
Knoxville TN

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