RE: [Histonet] RE: Retic jargon - Wiki

From:"Monson, Frederick "

Further, if we don't know more than is given on Wikipedia, we deserve to
believe what is written there as Gospel.

Frederick C. Monson, PhD
Technical Director
Microanalysis and Imaging Research and Training Center (MIRTC)
Large Scientific Instrument Core
Geology, West Chester University
S. Church St. and W. Rosedale Ave.
West Chester, PA, 19320
New Scheduler:
Web Page:


-----Original Message-----
[] On Behalf Of Renko,
Heather D.
Sent: Friday, November 30, 2007 1:39 PM
Subject: [Histonet] RE: Retic jargon

We say "Reticulum" ??? 
The term reticulin was coined in 1892 by M. Siegfried.[2]
Today the term reticulin or reticular fiber is restricted to fibers
composed of type III collagen  .
However, during the pre-molecular era, there was confusion in the use of
the term 'reticulin', which was used to describe two structures:
*	the argyrophilic (silver staining) fibrous structures present in
basement membranes   
*	histologically similar fibers present in developing connective
tissue[3]  . 
The history of the reticulin silver stain is reviewed by Puchtler et al.
(1978).[4]   The abstract of this paper
		Maresch (1905) introduced Bielschowsky's silver
impregnation technic for neurofibrils as a stain for reticulum fibers,
but emphasized the nonspecificity of such procedures. This lack of
specificity has been confirmed repeatedly. Yet, since the 1920's the
definition of "reticulin" and studies of its distribution were based
solely on silver impregnation technics. The chemical mechanism and
specificity of this group of stains is obscure. Application of Gomori's
and Wilder's methods to human tissues showed variations of staining
patterns with the fixatives and technics employed. Besides reticulum
fibers, various other tissue structures, e.g. I bands of striated
muscle, fibers in nervous tissues, and model substances, e.g.
polysaccharides, egg white, gliadin, were also stained. Deposition of
silver compounds on reticulum fibers was limited to an easily removable
substance; the remaining collagen component did not bind silver. These
histochemical studies indicate that silver impregnation technics for
reticulum fibers have no chemical significance and cannot be considered
as histochemical technics for "reticulin" or type III collagen.

 Who'd a thunk it?  TGIF

Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410

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