RE: [Histonet] Nissl staining question: ignorant question from an ignorant person

From:"Smith, Allen"

My cytology professor, Seymour Gelfant, recommended toluidine blue as a Nissl stain.  Lillie's HISTOPATHOLOGIC TECHNIC also says that toluidine blue can be used as a Nissl stain.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
From: [] On Behalf Of Sharon
Sent: Thursday, December 06, 2007 5:42 PM
Subject: [Histonet] Nissl staining question: ignorant question from an ignorant person

Dear Histonetters,

I have some questions about Nissl staining - (I have looked these
issues up in histology texts but am still confused because I have
received contradictory information - please clarify)
Here are my ridiculous questions:
1.  Is crystal violet ever used to stain epon embedded tissue for
pathology?  (I don't think so)
2.  Is toluidine blue used as a Nissl stain? (I think so)
3.  Are there advantages to Nissl staining with Cresyl Violet vs.
Toluidine blue? (when you are just looking for pathology with no
other stain - eg. not using the Toluidine blue or Cresyl Violet as a
counter stain for some other stain).
4. Is Cresyl Violet used for anything other than a Nissl stain in brain?

Thanks for your indulgence.


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