RE: [Histonet] Mast cell identification

From:"Houston, Ronald"

Not with Dako's antibody, but have used Novocastra's (cat# NCL-MCTRYP)
at dilution of 1:640 after EDTA retrieval

Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Columbus Children's Hospital is now Nationwide Children's Hospital

-----Original Message-----
[] On Behalf Of Cindy
Sent: Thursday, December 13, 2007 11:07 AM
To: histonet
Subject: [Histonet] Mast cell identification

Does anyone have experience using DAKO antibody-Mast Cell Tryptase?  We
have been using giemsa stains but my pathologist has asked me to look
for something more definitive to identify and stain mast cells.  If
anyone could offer advice I would appreciate it.
Cindi Robinson, HT (ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes, SD  57049
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