Jeannette,

We had a similar problem and solved it by switching to non-charged slides for our silver stains.  It seems that the adhesive coating was taking up the stain.

Best of Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mitchell,
Jeannette M.
Sent: Monday, December 03, 2007 3:27 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] H Pylori



Has anyone ever had dirty staining in negative gastric comtrol?  I am getting some nonspecific staining in some of my gastric biopsies.  The nonspecific staining I am talking about is groups of ppt., almost looks like bacteria but is way to big and is not uniform in size or clumps.  I don't see it in any of the other Ipexes that we are currently performing just the gastric biopsies.  Any ideas anyone??

Jeannette Mitchell, BS, HTL(ASCP), QIHC
R&D Histotechnologist
EP1-119
Fletcher Allen Health Care
Burlington, VT 05403


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