[Histonet] re:Problems in Neurofilaments-31 Staining

From:"Carl Hobbs"



Hi.
If you want to demonstrate NFs in general, I am surprised that you have 
chosen to test an Ab reagent that has not been validated for pwax sections, 
by Abcam.
It may well be that this SMI31 clone is not pwax-reactive.
If I am wrong..my apologies.
Sigma's anti NF 68 (N-5139), NF160 ( N-5264), NF200 ( N-0142) alone or in a 
cocktail are very good.
 Also, "RMO" clone (NF160) and RT97 clone ( NF200) are superb Ab reagents.
Imho, these are the "standards" for Neurofilament detection.
Carl


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