If you want to demonstrate NFs in general, I am surprised that you have
chosen to test an Ab reagent that has not been validated for pwax sections,
It may well be that this SMI31 clone is not pwax-reactive.
If I am wrong..my apologies.
Sigma's anti NF 68 (N-5139), NF160 ( N-5264), NF200 ( N-0142) alone or in a
cocktail are very good.
Also, "RMO" clone (NF160) and RT97 clone ( NF200) are superb Ab reagents.
Imho, these are the "standards" for Neurofilament detection.
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