Firstly: As a matter of interest - when using small amounts of primary
antibody does anyone use coverslips to spread the solution over the section
and keep it from evaporating during the incubation? (i do use a humidity
chamber also). I have been doing this for ages and wondering if there is an
alternative to use other than the coverslips that may be cheaper - any
secondly, Although 30min incubations are recommended, is there any value to
increasing incubation times of secondary and ABC complexes to improve
sensitivity? In other words is there a maximal binding period after which no
improvement will be noted?
I hope I have made myself clear here....these are just passing wednesday
thoughts in preparation for the usual friday meltdown!
Bone Research Unit
University of the Witwatersrand
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