We have just received a frozen human hemisphere, presumably
frozen in dry ice. The patient had a somewhat rare disorder.
We want to transfer it to formalin to do IHC. We routinely
do IHC on these brains using free-floating formalin fixed sections.
Therefore, would have to freeze again to cut.
Question: Best way to do this while minimizing damage
1. Thaw slowly in cold (4C) formalin for a long time in order
to allow fix to penetrate?
2. Thaw quickly and then put in fix?
2. Try to block while frozen with a saw?
3. Other suggestions?
UCLA / VA Medical Center
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