I have some questions about Nissl staining - (I have looked these
issues up in histology texts but am still confused because I have
received contradictory information - please clarify)
Here are my ridiculous questions:
1. Is crystal violet ever used to stain epon embedded tissue for
pathology? (I don't think so)
2. Is toluidine blue used as a Nissl stain? (I think so)
3. Are there advantages to Nissl staining with Cresyl Violet vs.
Toluidine blue? (when you are just looking for pathology with no
other stain - eg. not using the Toluidine blue or Cresyl Violet as a
counter stain for some other stain).
4. Is Cresyl Violet used for anything other than a Nissl stain in brain?
Thanks for your indulgence.
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