I have nothing but the highest regard for Leica sales, equipment and service.  They have some of the most dependable equipment on the market and are second to none in reponsiveness. To advise people not to buy their products before contacting the main company is wrong.  
   
  Louri Roberts
   
  Director Client Services
  Billing Consultants, LLC
  www.billingconsultants.net
   
   
  

histonet-request@lists.utsouthwestern.edu wrote:
  Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

1. bone saws (Stacy McLaughlin)
2. RE: 10% formaldehyde?? (Burrill, Jason)
3. RE: bone saws (Mike Pence)
4. RE: Re: Pregnancy (Burton, Lynn)
5. WARNING ABOUT LEICA MICROTOMES AND SERVICE and Suggestions
needed (Robert Schloesser)
6. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed (Jan.Minshew@leica-microsystems.com)
7. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed (Colleen Forster)
8. Leica microtomes, service and suggestion (Gayle Callis)
9. RE: WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed (MARY T HODGES)
10. AW: [Histonet] kappa and lambda BM ISH (Gudrun Lang)
11. Re: Leica microtomes, service and suggestion
(pmarcum@vet.upenn.edu)
12. Immunofluorescence (Cheryl Crowder)
13. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed (Rene J Buesa)
14. RE: WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed (Jack Ratliff)
15. Re: Immunofluorescence (Rene J Buesa)
16. Re: WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed (Robert Schloesser)
17. Re: Immunofluorescence (koellingr@comcast.net)


----------------------------------------------------------------------

Message: 1
Date: Fri, 14 Dec 2007 14:03:25 -0500
From: "Stacy McLaughlin" 
Subject: [Histonet] bone saws
To: 
Message-ID:


Content-Type: text/plain; charset="iso-8859-1"

Hi,
I'm looking for information on bone saws. It will primarily be used for femoral heads and knee caps. What kinds are you using?
My Pathologist wants some kind of device that will immobilize the femoral heads so they don't roll around when cutting. Any ideas out there?
As always your answers are appreciated!
Stacy McLaughlin, HT
Cooley Dickinson Hospital


------------------------------

Message: 2
Date: Fri, 14 Dec 2007 14:07:09 -0500
From: "Burrill, Jason" 
Subject: [Histonet] RE: 10% formaldehyde??
To: , 
Message-ID:
<1AD4E907E9B6F648AEF1B3A20A9B0E1E8F0ECE@shr-exch2.na01.crl.com>
Content-Type: text/plain; charset="koi8-r"

Dear Sara,



According to the Dangerous Goods Regulations 10% formalin (4%formaldehyde) is not regulated by either the DOT or IATA.š The cut off for the percentage of formaldehyde in a solution that is considered a Dangerous Good and must be classified is ™10% formaldehyde.š 



So since 10% formalin is only 4% formaldehyde it is not regulated as a Dangerous Good but 10% formaldehyde is considered a "Aviation regulated liquid, n.o.s. UN3334" and subject to classification when shipping by air.š This is a common mistake for people who ship samples fixed in 10% formalin.š If you would like to discuss this further or would like additional references to confirm this please call or e-mail me.



Regards,

Jason





Jason Burrill

Manager, Histology

Charles River Laboratories

251 Ballardvale Street

Wilmington, MA 01887

Ph: 781-222-6152

Fax: 978-988-8793

jason.burrill@crl.com 

**Please note new direct dial telephone number**





------------------------------

Message: 3
Date: Fri, 14 Dec 2007 13:18:54 -0600
From: "Mike Pence" 
Subject: RE: [Histonet] bone saws
To: "Stacy McLaughlin" ,

Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C809@IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"

This is the saw we use for just that purpose.
http://www.thermo.com/com/cda/product/detail/1,,10121267,00.html

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy
McLaughlin
Sent: Friday, December 14, 2007 1:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone saws


Hi,
I'm looking for information on bone saws. It will primarily be used for
femoral heads and knee caps. What kinds are you using? My Pathologist
wants some kind of device that will immobilize the femoral heads so they
don't roll around when cutting. Any ideas out there? As always your
answers are appreciated! Stacy McLaughlin, HT Cooley Dickinson Hospital
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 4
Date: Fri, 14 Dec 2007 13:21:28 -0600
From: "Burton, Lynn" 
Subject: RE: [Histonet] Re: Pregnancy
To: "Laurie Popp" ,

Message-ID:

Content-Type: text/plain; charset="iso-8859-1"

My children are now 13,10, and almost 6 and my experience was the same. My last 2 children were higher risk because I was preeclamptic with the first and was diabetic with the last. I made a list in the lab of all the chemicals after I physically went through and read each MSDS. If anyone is interested I would happily fax it to you.
Merry Christmas!
Lynn Burton

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp
Sent: Thu 12/13/2007 5:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Pregnancy



Hi Histonetters,

I actually just had a healthy baby girl 8/10/07 and was a high risk
pregnancy ( diabetic) while finishing my HT and working full time in the
histo lab at Mayo. I was advised by my OB to limit my formalin
exposure, to limit my xylene exposure as much as possible, and to be
very careful in special stains area. To also be careful of B-5 which we
use for our bone marrows and the list goes on. Basically I was limited
to cutting and embedding most days.

Hope this helps!

Laurie Popp, BA HT ( ASCP)
and happy mommy to Kaiana :-)


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 5
Date: Fri, 14 Dec 2007 16:58:50 -0500
From: Robert Schloesser 
Subject: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed
To: 
Cc: service@leica-microsystems.com,
"Ernie.Oates@leica-microsystems.com"
,
Tino.Thakral@leica-microsystems.com
Message-ID: 
Content-Type: text/plain; charset="US-ASCII"

Hi all Histotechs, 

We have a LEICA SM2000 R microtome. It worked fine for a few years but
recently broke (the stage would not move anymore). We spend $2000 and over 3
month trying to get it repaired by LEICA. LEICAs customer service is
completely incompetent and its technicians are even more incompetent (they
came our more than 6 times - they even send someone from Chigaco here to DC
to try to repair a simple microtome).

Anyways, to make a sad story short I would STRONGLY advice everyone not to
buy ANY products from LEICA that might require service.

Furthermore, I would like to hear from you if there is a suggestion from the
community what microtome to buy (since we have decided to throw our LEICA
SM2000 in the trash). We need a microtome for cutting frozen brain tissue
from rodents and primates. (usually 40um thick, sometimes up to 300um,
sometimes thinner). We usually use STURKEY MICROTOME KNIFES.

Any suggestions? Thank you

Robert


Robert J. Schloesser,MD
visiting research fellow
DHHS/NIH/NIMH/MAP/LMP

Laboratory of Molecular Pathophysiology (LMP)
Mood and Anxiety Disorders Program (MAP)
National Institute of Mental Health (NIMH)

Building 35/1C-912
35 Convent Drive
Bethesda, MD 20892-3711
Phone: 301-451-8435
FAX: 301-480-0123




On 12/14/07 2:21 PM, "Burton, Lynn" wrote:

> My children are now 13,10, and almost 6 and my experience was the same. My
> last 2 children were higher risk because I was preeclamptic with the first and
> was diabetic with the last. I made a list in the lab of all the chemicals
> after I physically went through and read each MSDS. If anyone is interested I
> would happily fax it to you.
> Merry Christmas!
> Lynn Burton
> 
> ________________________________
> 
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp
> Sent: Thu 12/13/2007 5:56 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Pregnancy
> 
> 
> 
> Hi Histonetters,
> 
> I actually just had a healthy baby girl 8/10/07 and was a high risk
> pregnancy ( diabetic) while finishing my HT and working full time in the
> histo lab at Mayo. I was advised by my OB to limit my formalin
> exposure, to limit my xylene exposure as much as possible, and to be
> very careful in special stains area. To also be careful of B-5 which we
> use for our bone marrows and the list goes on. Basically I was limited
> to cutting and embedding most days.
> 
> Hope this helps!
> 
> Laurie Popp, BA HT ( ASCP)
> and happy mommy to Kaiana :-)
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 6
Date: Fri, 14 Dec 2007 16:21:55 -0600
From: Jan.Minshew@leica-microsystems.com
Subject: Re: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed
To: Robert Schloesser 
Cc: histonet@lists.utsouthwestern.edu, service@leica-microsystems.com,
histonet-bounces@lists.utsouthwestern.edu,
"Ernie.Oates@leica-microsystems.com"
,
Tino.Thakral@leica-microsystems.com
Message-ID:


Content-Type: text/plain; charset=US-ASCII

Hello Dr. Schloesser,

I am the Marketing Manager for the Leica SM2000 R and I would like to
assure you that I was unaware of your difficulties. Please feel free to
contact me so I can help resolve the obvious frustration you have
encountered with the servicing of this instrument.

Best wishes,


Jan Minshew, HT/HTL(ASCP)
Marketing Manager
Leica Microsystems
Biosystems Division
2345 Waukegan Road
Bannockburn, IL 60015

800.248.0123 Toll Free
847.405.7051 Direct
847.405.6560 Fax

www.leica-microsystems.com

Click Here for this month's special offers!



Robert Schloesser 
h.gov> To 
Sent by: 
histonet-bounces@ cc 
lists.utsouthwest service@leica-microsystems.com, 
ern.edu "Ernie.Oates@leica-microsystems.com 
" 
12/14/2007 03:58 >, 
PM Tino.Thakral@leica-microsystems.com 
Subject 
[Histonet] WARNING ABOUT LEICA 
MICROTOMES AND SERVICE and 
Suggestions needed 










Hi all Histotechs,

We have a LEICA SM2000 R microtome. It worked fine for a few years but
recently broke (the stage would not move anymore). We spend $2000 and over
3
month trying to get it repaired by LEICA. LEICAs customer service is
completely incompetent and its technicians are even more incompetent (they
came our more than 6 times - they even send someone from Chigaco here to DC
to try to repair a simple microtome).

Anyways, to make a sad story short I would STRONGLY advice everyone not to
buy ANY products from LEICA that might require service.

Furthermore, I would like to hear from you if there is a suggestion from
the
community what microtome to buy (since we have decided to throw our LEICA
SM2000 in the trash). We need a microtome for cutting frozen brain tissue
from rodents and primates. (usually 40um thick, sometimes up to 300um,
sometimes thinner). We usually use STURKEY MICROTOME KNIFES.

Any suggestions? Thank you

Robert


Robert J. Schloesser,MD
visiting research fellow
DHHS/NIH/NIMH/MAP/LMP

Laboratory of Molecular Pathophysiology (LMP)
Mood and Anxiety Disorders Program (MAP)
National Institute of Mental Health (NIMH)

Building 35/1C-912
35 Convent Drive
Bethesda, MD 20892-3711
Phone: 301-451-8435
FAX: 301-480-0123




On 12/14/07 2:21 PM, "Burton, Lynn" wrote:

> My children are now 13,10, and almost 6 and my experience was the same.
My
> last 2 children were higher risk because I was preeclamptic with the
first and
> was diabetic with the last. I made a list in the lab of all the chemicals
> after I physically went through and read each MSDS. If anyone is
interested I
> would happily fax it to you.
> Merry Christmas!
> Lynn Burton
>
> ________________________________
>
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Popp
> Sent: Thu 12/13/2007 5:56 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Pregnancy
>
>
>
> Hi Histonetters,
>
> I actually just had a healthy baby girl 8/10/07 and was a high risk
> pregnancy ( diabetic) while finishing my HT and working full time in the
> histo lab at Mayo. I was advised by my OB to limit my formalin
> exposure, to limit my xylene exposure as much as possible, and to be
> very careful in special stains area. To also be careful of B-5 which we
> use for our bone marrows and the list goes on. Basically I was limited
> to cutting and embedding most days.
>
> Hope this helps!
>
> Laurie Popp, BA HT ( ASCP)
> and happy mommy to Kaiana :-)
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________



------------------------------

Message: 7
Date: Fri, 14 Dec 2007 17:15:50 -0600
From: Colleen Forster 
Subject: Re: [Histonet] WARNING ABOUT LEICA MICROTOMES AND SERVICE and
Suggestions needed
To: Jan.Minshew@leica-microsystems.com
Cc: histonet@lists.utsouthwestern.edu,
Tino.Thakral@leica-microsystems.com, service@leica-microsystems.com,
histonet-bounces@lists.utsouthwestern.edu,
"Ernie.Oates@leica-microsystems.com"

Message-ID: <47630EA6.30405@umn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

To Dr.Schloesser,

Although I am sorry to hear that you have had trouble with your Leica 
rep. I have to say in their defense I have a very good one. They are 
always prompt to answer my phone calls and have always provided the 
service and support I need. I think it would have been better if you had 
contacted the main Leica company BEFORE you attempted to roast them on 
the histonet as Leica has a very good product and is a very reputable 
company.

My 2 cents for the day!!

Colleen Forster
U of MN


Jan.Minshew@leica-microsystems.com wrote:
> Hello Dr. Schloesser,
>
> I am the Marketing Manager for the Leica SM2000 R and I would like to
> assure you that I was unaware of your difficulties. Please feel free to
> contact me so I can help resolve the obvious frustration you have
> encountered with the servicing of this instrument.
>
> Best wishes,
>
>
> Jan Minshew, HT/HTL(ASCP)
> Marketing Manager
> Leica Microsystems
> Biosystems Division
> 2345 Waukegan Road
> Bannockburn, IL 60015
>
> 800.248.0123 Toll Free
> 847.405.7051 Direct
> 847.405.6560 Fax
>
> www.leica-microsystems.com
>
> Click Here for this month's special offers!
>
>
> 
> Robert Schloesser 
> > h.gov> To 
> Sent by: 
> histonet-bounces@ cc 
> lists.utsouthwest service@leica-microsystems.com, 
> ern.edu "Ernie.Oates@leica-microsystems.com 
> " 
> > 12/14/2007 03:58 >, 
> PM Tino.Thakral@leica-microsystems.com 
> Subject 
> [Histonet] WARNING ABOUT LEICA 
> MICROTOMES AND SERVICE and 
> Suggestions needed 
> 
> 
> 
> 
> 
> 
>
>
>
>
> Hi all Histotechs,
>
> We have a LEICA SM2000 R microtome. It worked fine for a few years but
> recently broke (the stage would not move anymore). We spend $2000 and over
> 3
> month trying to get it repaired by LEICA. LEICAs customer service is
> completely incompetent and its technicians are even more incompetent (they
> came our more than 6 times - they even send someone from Chigaco here to DC
> to try to repair a simple microtome).
>
> Anyways, to make a sad story short I would STRONGLY advice everyone not to
> buy ANY products from LEICA that might require service.
>
> Furthermore, I would like to hear from you if there is a suggestion from

=== message truncated ===

       
---------------------------------
Be a better friend, newshound, and know-it-all with Yahoo! Mobile.  Try it now.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet