[Histonet] Interrupted thionin staining

From:"Nicola J. Broadbent Ph.D"


I would like to know whether it is ok to place rat brain tissue (50um slices
on glass gelatin coated slides) directly from xylene to 100% alcholol to
start the thionin staining process.  Normally, we defat in a mix of
chloroform and alcohol (approx 1 hr; which we did do with these brain
sections) but due to being evacuated from our building we had to stop the
staining process and in to store the tissue (as we were unsure how long we
would be away from the lab) we placed the tissue in the zylene.  The
sections ended up being in zylene for approx 24 hrs.  Would it be ok just to
start the staining process from this point i.e. go straight to the 100%, 95%
70% alcohol steps from the zylene?

Thanks so much for your help,

Nicola J. Broadbent Ph.D,
Asst. Project Scientist
Department of Psychiatry 0603,
UCSD School of Medicine,
9500 Gilman Dr,
La Jolla, CA, 92093-0603

Email: nbroadbent@ucsd.edu
Phone: (858) 642 3628 or 
           (858) 552 8585 x 7853
Fax: (858) 552 7457 

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