[Histonet] Renal bxs for IF

From:"Chaussey, Leslie"

Hello -

Just wondering how various labs out there are addressing the CAP reg below.  Thanks for any input in advance.


Are appropriate positive and negative controls performed with each case tested using immunofluorescence? 

NOTE:  Internal antigens serve as positive controls (e.g., IgA in tubular cases, IgG in protein droplets, and C3 in blood vessels).  Non-reactive elements in the patient specimen may serve as a negative tissue control.  A negative reagent control in which the patient tissue is processed in an identical manner to the test specimen but with the primary antibody omitted must be performed for each patient test specimen.

Reference:  Walker PD, et al. Practice guidelines for the renal biopsy.  Mod. Pathol. 2004;17:1555-1563.


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