[Histonet] MGMT
Dear Histonetters,
I have been trying to get the antibody MGMT (
O6-Methylguanine-DNA-Methyltransferase) to work on a Ventana Benchmark
system. I have one control that appeared to work but I have not found
any other tissue that has stained. I feel I need to tweak this antibody
some perhaps I am getting false negative reactions. I have been using
Lab Vision Neomarkers antibody at 1:50 with mild CC1 epitope retrieval
with amplifier. (using i-view dab detection)
Any comments may be helpful
Elaine Dooley
HTL
Shands Teaching Hosp.
Gainesville FL
352-265-0111 ext 72117
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