[Histonet] GMS Precipitant

From:"McGraw, Medea J"

I had a question concerning the Gordon and Sweets Retic stain. I have been getting great results with the fibers picking up the silver quite nicely; however, there are areas within the tissue that have a granular look to them. It looks like the silver precipitated into the surrounding tissue and sort of can interfere with reading out the slides. I am trying to "clean up" the stain and was wondering if you had any suggestions. I have been blocking the sections prior to staining with 0.1% NFR and also a quick counterstain at the end with NFR and it looks great and mostly clean except for these granules. Do you suggest possibly adding more time/ increasing the % of Sodium Thio or would you suggest possibly trying a new reagent of silver. I suspect that the silver  that we are using might not be the best quality. If you have any suggestions it would be much appreciated. Thanks and happy holidays.

Medea J. McGraw , BS, HT, HTL (ASCP) 
SCCA Pathology Department
Desk: 206-288-1358
Main Lab: 206-288-1355
Email: mmcgraw@seattlecca.org

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