Re: [Histonet] paraffin problem

From:Rene J Buesa

  In case you want to keep a score of answers/preferences, I alwys used Paraplast (Plus for infiltraiton, and Extra for embedding).
  René J.

"Vickroy, Jim"  wrote:

Lately we are experiencing a problem with our paraffin blocks. We use
Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon
very well and at times the sections have some small striations, almost
like knife lines. We have checked the disposable knives and have
eliminated that variable. We use a recycled clearing agent but have
eliminated that also by using fresh chemicals. We have several tissue
processors and there is a possibility that the problem could be a
processor issue however the majority of us think that it is a paraffin
problem. We do not see any "grit" in the paraffins. We are thinking of
trying a different paraffin and wondered if anyone has switched to one
of the newer paraffins that work great. Obviously I can get as many
samples as I would like but want to see if anyone has any suggestions or
ideas that would help us eliminate some of the steps of the process.

Jim Vickroy

Technical Supervisor - Surgical and Autopsy Pathology

Memorial Medical Center

This message (including any attachments) contains confidential information intended for a 
specific individual and purpose, and is protected by law. If you are not the intended recipient, 
you should delete this message. Any disclosure, copying, or distribution of this message, or the 
taking of any action based on it, is strictly prohibited.
Histonet mailing list

Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster.
Histonet mailing list

<< Previous Message | Next Message >>