Re: [Histonet] control block for Pseudomonas aeruginosa

From:"Vinnie Della Speranza"

Hi Liz,
I haven't seen a reply to your question. I'm offering this information in the hope that it may offer a compromise solution to your need.

American Type Culture Collection ( ATCC),  can provide you with a live culture of pseudomonas aeruginosa. keep in mind my last dealings with them was pre-9/11 so I don't know if this is as easy as it used to be.

if you have access to a microbiology lab they should be able to grow this bug in a broth solution to prepare a sufficient volume that you could perfuse into fresh lung. my recollection is that this bug is not fastidious but I do recommend that you enlist the aid of those with BioSafety level II capability. 

I'm less certain of the difficulty in obtaining cystic fibrosis lung tissue and do not know of any commercial source for this tissue.

good luck,

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> "Liz Chlipala"  11/29/06 03:54PM >>>
Hello All


I need to locate a block of human Cystic Fibrosis lung positive for
P.aeruginosa.  Does anyone know where I could get something like this.


Thanks in advance




Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC


Premier Laboratory, LLC

P.O. Box 18592

Boulder, CO 80308

phone (303) 735-5001

fax (303) 735-3540 


Ship to Address:


Premier Laboratory, LLC

University of Colorado at Boulder

MCDB, Room A3B40

Boulder, CO 80309


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