Re: [Histonet] ICAM-1 Antibody

From:Gayle Callis

The KAT-1 clone should work (Serotec has this also).  We would use BD 
Pharmingens Armenian hamster antiMouse CD54 (iCAM), negative control would 
be Armenian hamster IgG, Group1, kappa as the negative IgG control. BD 
Pharmingen has a photo available in catalog and probably on website.  This 
antibody will bind more than just vascular tissue, it also detects 
lymphocytes, high endothelial venules (HEV), epithelial cells, macrophages 
and dendritic cells.

Beware, you may NOT get staining after paraformaldehyde fixation.  Read the 
data sheet from the website for fixation recommendations.

Acetone 4C for 10 min should work, and for better morphology, acetone 75 ml 
/ absolute ethanol 25 ml, fix overnight air dried frozen sections from 
fresh tissue for 5 min at room temperature, then go directly from this 
fixative to rinse buffer, 3 changes.

At 06:45 PM 12/7/2006, you wrote:
>I need information about good purified rat anti mouse ICAM-1 monoclonal 
>antibody. I want to buy from Chemicon but there are apparently two types 
>of clones from Chemicon for rat antimouse ICAM-1. CLone KAT-1 and Clone 
>LTF653. Does anyone has any experience on using this antibody ?
>I need to stain mouse vascular tissue on frozen section with 4% 
>Paraformaldehyde fixation. Thanks a lot for the help.
>Wulan Anggrahini
>Kobe University
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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