Re: [Histonet] H&E slides fading in a few days

From:Rene J Buesa

  The answer is in your question: chlorine is the culprit!
  You can decolorize ANY stain with chlorine. You will have to eliminate the chlorine and one of the most effective ways is to heat the water.
  Ren J.

"Yeung, Iris [VA]"  wrote:

We have problem with the slides fading in a relatively short time. Our
water supply is high in chlorine. We use Gill's III and alcoholic eosin.
Coverslip in Entellin(?spelling) and glass coverslip. Seems to fade in
small bx like prostatic needle bx than larger blocks. Any suggestion why
it happens??

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